HF®, Nicking, Master Mix, Time-Saver & Other, Restriction Endonuclease Buffers & Diluents, DNA Modifying Enzymes & Cloning Technologies, DNA Assembly, Cloning and Mutagenesis Kits, Protein Expression & Purification Technologies, NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®), NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™), Recombinant Albumin, Molecular Biology Grade, Reduce Star Activity with High-Fidelity Restriction Enzymes, Standard Protocol for Restriction Enzyme Digests. See more details on Bioz. Additional features include sgRNA Template Oligo Design and qPCR library quantification. At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction-modification systems. NEBcloner®. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. Read More > BbsI We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Use Enzyme Finder to select a restriction enzyme by category, recognition sequence, overhang or name to find information on that enzyme. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. Browse NEB's Restriction Enzyme Digestion Products. Also does virtual digestion. Use this tool to select restriction enzymes by name, sequence, overhang or type. You have been idle for more than 20 minutes, for your security you have been logged out. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job. Learn more about how Type II REs work. Home Tools & Resources Video Library NEB Restriction Enzyme Double Digest ... it's simply adding your two enzymes together, at a ratio of 5 to 10 units of enzyme per microgram of DNA, adding the CutSmart Buffer, bringing the volume to ... but have a different buffer, simply go to our website, use the Double Digest Finder application. Typically, … Is this enzyme sensitive to dam, dcm or mammalian CpG methylation? To learn more and manage cookies, please refer to our Cookie Statement. Use this tool to help select the right DNA polymerase for your PCR setup. Use this tool to select restriction enzymes by name, sequence, overhang or type. Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence; Cut Site: ACTGG(1/-1) Powered by Bioz. All information on the website has been updated to reflect this change. For example, in the above image applying a BTG enzyme first will not cleave any β galactose until all Neu5ac, Neu5Gc and α galactose are first removed Close. All information on the website has been updated to reflect this change. For maximum convenience and value, columns and buffers are also available separately. Choose a DNA, RNA, genome editing, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. NEB LAMP Primer Design Tool can be used to design primers for your Loop-mediated Isothermal Amplification. Take advantage of free shipping for any order totaling over $350. AatII AbaSI Acc65I AccI AciI AclI AcuI ... To save your cart and view previous orders, sign in to your NEB account. You can browse NEB's tools and resources below by resource type, product category or application. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Use the NEBuilder Assembly Tool to design primers for your HiFi DNA or Gibson Assembly reactions, based on sequences and polymerase used for amplification. 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Testing at NEB has shown that there is no difference in activity and stability between enzymes that are shipped with ice packs and enzymes shipped on dry ice. Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. Use the NEBuilder Assembly Tool to design primers for your HiFi DNA or Gibson Assembly reactions, based on sequences and polymerase used for amplification. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction. Use Enzyme Finder to select a restriction enzyme by category, recognition sequence, overhang or name to find information on that enzyme. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. Determine buffer and reaction conditions for experiments requiring two restriction enzymes using the Double Digest Finder. We use cookies to understand how you use our site and to improve the overall user experience. Enzyme Finder Use this tool to select restriction enzymes by name, sequence, overhang or type. Whether you are quickly screening large numbers of clones or setting up overnight digests, you will benefit from the high quality of our enzymes. BbsI-HF ®. NEBtools brings New England Biolabs’ most popular web tools to your phone, allowing you to plan your experiments from anywhere. Software screenshots are marked in grey boxes. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. Synthetic Biology/DNA Assembly Selection Chart; DNA Ligase Selection Chart; Isoschizomere der Restriktionsenzyme; Troubleshooting Guides. Double digests with NEB's restriction enzymes can be set up in CutSmart Buffer. Please review the enzyme list and recommendations in the following table as well as the background information article. Find out from an NEB scientist. Contact your local subsidiary or distributor. For Help With Your Order. Help - Building the exoglycosidase panel. email or call 1-800-NEB-LABS. IPSWICH, MASSACHUSETTS (November 1, 2011) New England Biolabs (NEB), a world leader in the production and supply of reagents for the life science industry, has made its popular web tools, Double Digest Finder and Enzyme Finder, available as the "NEB Tools" app for Android™ phones and tablets. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)? Use this tool to guide you through selection of NEBNext reagents for next generation sequencing sample preparation. To Request Technical Support. international site. dna modifying enzymes (cloning) dna polymerases (cloning) epigenetics; genome editing; glycomics; ngs sample prep; nucleic acid purification; nucleic acids (cloning) other products; other products (cloning) phage display (phd) protein analysis, exp & purification; restriction enzymes; rna; synthetic biology Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Fill out our Technical Support Form, email us, or call 1-800-632-7799. Your profile has been mapped to an Institution, please sign back for your profile updates to be completed. Otherwise, choose an NEBuffer that results in the most activity for both enzymes. NEBtools brings New England Biolabs’ most popular web tools to your phone, allowing you to plan your experiments from anywhere. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job. What is the activity of the Type IIS restriction enzyme BsaI-HFv2 (NEB #R3733) in T4 DNA Ligase Buffer? Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme … Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance. Isoschizomers | Single Letter Code Product Introduction. Isoschizomers | Single Letter Code Product Introduction. Are you doing COVID-19 related research? Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. View our selection of online tools currently in development. EcoRI-HF® We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job. No non-base letters. Contact our Customer Service Team by email or call 1-800-NEB-LABS. Here are some tips to help you determine the cause. email us, or call 1-800-632-7799. NEBtools brings New England Biolabs’ most popular web tools to your iPhone and iPad, allowing you to plan your experiments from anywhere. Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Info: Double Digests. This includes personalizing content and advertising. enzymes of the highest purity and unparalleled performance. Use this tool to select restriction enzymes by name, sequence, overhang or type. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches. ... and this tool will identify the recommended NEB product. In addition, Double Digest Finder and Enzyme Finder are featured on NEBTools, our free app for iPhone ® and Android™. All information on the website has been updated to reflect this change. Set up reaction according to recommended protocol. With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of For Customers … Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence; Supplied with 1 vial of Gel Loading Dye, Purple (6X) Cut Site: GCATC(5/9) Powered by Bioz. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins. NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5 Site-Directed Mutagenesis Kit. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic. You have been idle for more than 20 minutes, for your security you have been logged out. DNA Assembly, Cloning and Mutagenesis Kits, Protein Expression & Purification Technologies, Thermostable Ligase Reaction Temperature Calculator. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Otherwise, choose an NEBuffer that results in the most activity for both enzymes. Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly. When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers. Let an NEB scientist help you troubleshoot your reaction. Choose Product: ... How will Time-Saver™ qualified enzymes save you time? restriction endonucleases, in DNA sequences. Are you finding unexpected bands in your digestion reaction? This animation discusses three guidelines for determining … How will Time-Saver™ qualified enzymes save you time? To save your cart and view previous orders, sign in to your NEB account. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Contact your local US Sales Representative. Monarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Info: Restriction Enzyme Tips. Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Browse NEB's 18 interactive tools, including Double Digest Finder, Enzyme Finder, NEBNext Selector, and NEBcloner. We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Reduce Star Activity with … Setting up a Double Digestion. Featured Video. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Learn more about these poorly understood enzymes. Please sign back in to continue your session. If you don't see your country above, please visit our NEB Golden Gate Assembly Tool; Double Digest Finder; NEB Cutter; DNA Sequences and Maps Tool; Enzyme Finder; Selection Tools. If star activity is a concern, consider using one of our High Fidelity (HF®) enzymes. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Use this tool to select restriction enzymes by name, sequence, overhang or type. NEBcutter V2.0 will indicate cut frequency and methylation state sensitivity. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Fill out our Technical Support Form, The tool guides you to product recommendations based on your answers to a few simple questions. EnGen sgRNA Template Oligo Designer can be used to design target-specific DNA oligos for use with the EnGen sgRNA Synthesis Kit. international site. Download Mobile apps for; Terms & Conditions; ISO certification; Privacy; Returns; Delivery Conditions; Disclaimer; Designed and developed by red transact Determine … Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. NEBNext Selector. Please sign back in to continue your session. Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. Time-Saver™ qualified for digestion in 5-15 minutes Enzyme Finder. Also, find other relevant tools and resources to enable protocol optimization. NEB only 5' overhang 3' overhang blunt Prototypes Only All Isoschizomers . Enzyme Finder. Not getting the cleavage you expected? being a leader in enzyme technologies. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment. For Questions Related to NEB Products and Offers. Are you doing COVID-19 related research? With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Learn how, and what this means for your digests. Troubleshooting. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA & RNA cleanup. NEBtools for Android also enables quick and easy access to NEB Technical Support by phone, email, or web when you need it. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance. Restriction Endonucleases; PCR, qPCR & Amplification Technologies; DNA Modifying Enzymes & Cloning Technologies; NGS Sample Prep & Target Enrichment; Nucleic Acid Purification Products; … Enzyme Finder. NEB’s online tools, NEBcloner and Double Digest Finder will help guide your reaction buffer selection when setting up double digests. Use Enzyme Finder to select a restriction enzyme by category, recognition sequence, overhang or name to find information on that enzyme. Download a PDF containing pricing for our full product list. Requires two or more sites for cleavage. All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency. ... Use this tool to select another company’s product and find out which NEB product is compatible. Blocked by some combinations of overlapping CpG methylation. … This tool designs probes to be used with the NEBNext RNA Depletion Core Reagent Set or to supplement an existing NEBNext Depletion Kit for the depletion of unwanted RNA species. Use this tool to select restriction enzymes by name, sequence, overhang or type. Are you doing COVID-19 related research? Time-Saver™ qualified for digestion in 5-15 minutes Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. NEB sells two neoschizomers of PleI. Double digests with NEB's restriction enzymes can be set up in CutSmart Buffer. Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. Our latest RUO kit, the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers. Why is My Restriction Enzyme Not Cutting DNA? Reduce Star Activity with High-Fidelity Restriction Enzymes View additional videos. Use this tool to interpret Ultra or High Pressure liquid chromatography (UPLC/HPLC) N-glycan profiles following exoglycosidase digestions. Find out from an NEB scientist. Also, find other relevant tools and resources to enable protocol optimization. All of NEB's Restriction enzymes have transitioned to a new buffer system. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. DNA Sequences & Maps Tools. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Methylation-sensitive restriction enzyme Your profile has been mapped to an Institution, please sign back for your profile updates to be completed. NEBcloner. Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers. Choose either the competitor’s product name or catalog number from the available selections, and this tool will identify the recommended NEB product. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings. Learn more and request a sample! NEB Tools for Restriction Enzyme Experiments Now Available for the Android Platform. Cloning With Restriction Enzymes Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. PleI leaves a 5´ overhang, MlyI is a blunt cutter while Nt.BstNBI is a nicking enzyme. Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Contact your local subsidiary or distributor. Save time and money by placing an order with NEB. Info: Optimizing Reactions. Watch as Geoff Wilson, Emeritus Scientist, describes what restriction enzymes are and how they revolutionized molecular biology. Read about our new 7-base recognition sequence Type IIS restriction enzyme and how it can be used for simple to complex 24-fragment Golden Gate Assembly, achieving our highest level of efficiency and fidelity yet. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions. Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases. Setting up a Double Digestion. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. If you don't see your country above, please visit our Contact our Customer Service Team by Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup. All information on the website has been updated to reflect this change. DpnI cleaves only when its recognition site is methylated. Learn more about how Type I REs work. The Leader in the Discovery and Production of Restriction Enzymes. Items in Your Cart: VIEW CART: NEB PRODUCTS. Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation. NEB has engineered HF® enzymes to eliminate star activity. Time-Saver™ qualified for digestion in 5-15 minutes Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8. By default, only enzymes available from NEB are used, but other sets may be chosen. Maps sites for restriction enzymes, a.k.a. Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. Place your order before 7:30pm EST for overnight delivery. To learn more and manage cookies, please refer to our Cookie Statement. Featured Video. Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. Download NEB Tools and enjoy it on your iPhone, iPad and iPod touch. Visit NEBCutSmart.com for further details. Choose either the competitor’s product name or catalog number from the available … Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. Info: RE Performance Chart. NEBcutter V2.0. Use this tool to simplify the process of selecting the appropriate exonucleases for use in your nucleic acid digestion workflows. Choose either the competitor’s product name or catalog number from the available … Otherwise, choose an NEBuffer that results in the most activity for both enzymes. Tool: NEBcutter-----Info: Troubleshooting Guide. NEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. Working continuously to be worthy of that distinction, NEB strives to develop Isoschizomers | Single Letter Code Product Introduction. Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. For added flexibility, NEB offers an isoschizomer or HF enzyme, supplied with CutSmart Buffer. These tools are not yet optimized for design and usability – we are looking for feedback on functionality and utility to improve them for future use. Some of our products are shipped at ambient temperature - find ... NEB enzymes are stored in buffered 50% glycerol and remain liquid at temperatures down to … Use this tool for your scientific calculations and conversions for DNA and RNA. NEB offers several helpful interactive tools for your research and experimental design. Choose between Type II and commercially available Type III restriction enzymes to digest your DNA. Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job. Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Double digests with NEB's restriction enzymes can be set up in CutSmart Buffer. Posted on Wednesday, April 7, 2021 By guest blogger Rebecca Kucera, M.S., NEB Principal Development Scientist. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors. version 1.8.1 ... Tool: Enzyme Finder. This tool allows for easy calculation of values associated with read coverage in NGS protocols. Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Restriction Enzyme Digest Problem: Too Many DNA Bands, Restriction Enzyme Digest Protocol: Cutting Close to DNA End, Optimizing Restriction Endonuclease Reactions. ... Use this tool to select another company’s product and find out which NEB product is compatible. Use NEBcutter2.0 tool to find the restriction sites within your DNA sequence, identifiying the sites for both Type II and comercially available Type III restriction enzymes. In December 2020 NEB UK introduced Eco-Boxes for cold shipment - find out more here. SfcI concentrations of < 1 unit/µg DNA in a reaction are not recommended. Use this tool to identify the restriction sites within your DNA sequence. Sequence Info. Determine buffer and rea… See more details on Bioz. Over 210 restriction enzymes are 100% active in a single buffer – CutSmart™ Buffer. Choose Product: Restriction Enzyme Digestion; Restriction Enzyme Digestion. NEB PRODUCTS. The final concentration of glycerol in any reaction should be less than 5% to minimize the possibility of star … Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. Use this tool to select another company's product and find out which NEB product is compatible. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. NEB’s online tools, NEBcloner and Double Digest Finder will help guide your reaction buffer selection when setting up double digests. ... NEB LAMP Primer Design Tool can be used to design primers for your Loop-mediated Isothermal Amplification. This focus allows us to provide extremely pure enzymes at concentrations that deliver more flexibility to your experimental design. We use cookies to understand how you use our site and to improve the overall user experience. Info: Dam-Dcm and CpG Methylation. The Leader in the Discovery and Production of Restriction Enzymes. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. SfcI is active for one hour in a reaction at 37°C, after one hour no further digestion takes place. Contact your local US Sales Representative. Enter your DNA sequence (maximun length 300KBases) and click on “submit” to find the restriction sites. Does NEB offer any BSA-free and/or animal origin-free restriction enzymes for linearization of plasmids for mRNA vaccine development? Use this tool as a guide to the ever-changing landscape of restriction enzymes. EcoRI-HF® We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. BsmBI-v2 We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. To save your cart and view previous orders, sign in to your NEB account. Fixed primers can be specified for the design of LAMP primers, and subsequent Loop primers are then designed based on LAMP primer selection.
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